| MUC7

Mucin-7 is a protein that is encoded by the MUC7 gene.[3][4] In animals, the MUC7 gene is found in most placental mammals, but not marsupials.Humans carry either a five or six tandem repeat version of the gene. In other primates, the number of repeats found is 4-5 for gorillas, 5 for chimpanzees, 6-7 for orangutans, 8-10 for macaques, 10-11 for baboons and 11-12 for green monkeys.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.28	1.24	0.67	0.35	0.19	0.14	0.11	0.09
Corrected OD	2.19	1.15	0.58	0.26	0.1	0.05	0.02	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human MUC7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Saliva(n=8)	84-101	96
Tissue Homogenates(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Human MUC7 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Saliva(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	85-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98